The altar painting of the Martyrs of Vilnius depicts three saints: Anthony (Kruglets), John (Kumets) and Eustathius (Nezhilo), executed by the Lithuanian Duke Olgerd (1345–1377), at whose court they had served, for the open confession of Christianity.
Oak and rope are depicted above the heads of the saints, a dagger and shackles symbolizing suffering and murder tools are depicted at their feet.
Subsequently, the oak, on which their execution had taken place, was cut down and the church in honour of the Holy Trinity was erected in its place. The Holy throne in the church was made of the trunk of an oak tree and the relics of the three holy martyrs were placed in it. Currently, the relics of the Vilnius saints are located in the Orthodox Church of the Holy Spirit in Vilnius.